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Putting a Foldable Microscope to the Test in the Amazon
A couple of months ago, I received an interesting package in the mail. It looked like a standard manila envelope, but inside was a device that could quite possibly revolutionize the way we view the microscopic world. I’m referring to the Foldscope, an origami-based optical microscope that is small enough to fit inside your pocket. The real kicker: the entire cost of the instrument is less than one dollar.
The Foldscope has received some recent and well-deserved media attention (the lab’s publication on this device recently made it in the top 20 papers in PlosOne for 2014) but I hadn’t seen many videos on the Foldscope being put to the test in the field. It seemed like there was a lot of potential for this invention but I wondered how it would fare on one of my expeditions through a jungle searching for unknown species. So I decided to assemble my miniature paper microscopes and travel to one of the most remote places in the world, the rainforest of the Peruvian Amazon, to give them a go.
The Results
Long story short, this device is amazing. During my time in the Amazon rainforest, I was able to investigate tiny insects, mites, fungi, and plant cells from 140x to 480x magnification without requiring a large and expensive conventional microscope.

The cells from a flower petal were recorded by connecting a cell phone to the Foldscope.
Some of the diverse arthropod specimens could potentially be new to science, so it was really exciting to document images and videos of these organisms right there in the field by connecting my phone to the Foldscope.

An unknown species of mite was documented by connecting a cell phone to the Foldscope.

A spider is infected by a parasitic fungus known as Cordyceps. The circles show regions of the fungus viewed under the Foldscope.
In the video, I investigate bizarre structures on a plant, which are known as leaf galls. These are sometimes created by insects, but they usually have to be taken back to a lab and inspected in detail under a microscope. Lucky for me, I had the Foldscope in the field! It turns out these were in fact due to insect larvae, which burrowed into the leaf and tweaked the chemistry of the plant to produce galls. Even with macro photography, I couldn’t get much detail of the larva, but at 140x magnification, under the Foldscope, I was able to document the morphology of this unknown critter.

Top left, a leaf is covered in galls. Top right, a cross-section of a gall; notice the tiny insect larva living inside! Bottom, the larva was placed under the Foldscope and viewed on my cell phone. Pictures and videos were recorded in real-time out in the Amazon rainforest.
Suspecting that the galls were formed by some sort of wasp or fly, I later got in touch with a couple of Diptera (fly) experts, Morgan Jackson (@BioInFocus) and Dr. Stephen Gaimari, who helped identify the gall-forming culprits as a possible species of fly belonging to the family Cecidomyiidae.
Final Thoughts
The research team, led by Dr. Manu Prakash, seeks to “democratize science” by developing tools that are able to scale up to match problems in global health and science education – and I believe they are doing just that with the Foldscope. This device is cheap, easy to use, and broadly applicable whether you’re a curious young student, a medical professional in the field, or someone who is interested in the numerous tiny things that surround us. Until now, I’ve never had a device that made viewing and sharing the microcosmos so accessible.

A closer look at the moss covering a tree in the tropical rainforest.

Morphology of a neotropical pseudoscorpion – all images were taken in the field with the Foldscope!

A breakdown of the unit costs for Foldscope components in volumes of 10,000 units, not including assembly costs (Cybulski, Clements, & Prakash 2014). The total cost of the Foldscope ranges from {%_CONTENIDO_%}.58 to {%_CONTENIDO_%}.97.
By Aaron Pomerantz
You can follow Aaron for more updates on Twitter @AaronPomerantz and the Stanford University Researchers @PrakashLab
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I can not say enough good things about my time at the Tambopata Research Center. Between the guides, the boat drivers, the chefs, the staff, the bartenders, the researchers, etc. Everyone was absolutely wonderful! If you are interested in viewing wildlife in the Amazon, this is the place for you! A special thank you to the maître d' Carlos who was so incredibly kind and hospitable and our guide Dino who was like a real life Indiana Jones. I can't wait to come back! Read all
An unforgettable, fully immersive jungle experience. Amazing how Tambopata Lodge delivers luxury touches in such a remote and rugged location. The food is fantastic, the staff incredible, and the wildlife sightings non-stop. The personalised itinerary and one-on-one guiding made the trip truly special—huge thanks to our guide Johan for sharing his passion and knowledge. The Macaw Project is inspiring, and it’s clear the lodge fully supports this important conservation work. Manager Brian was always kind, helpful. Well-done all involved. Read all
The most important fact we are grateful for is the true sense of understanding of the ecology of the Amazon the staff at Rainforest Expeditions (RFE) have shown us. RFE stands out for its stand against the pure, profit-motive initiatives many other tour operators do. We are very grateful to have had Carlos as our guide. Carlos is deeply dedicated, and is able to explain the nuances of the flora and fauna. The world today needs to act as one against the impact of climate change. RFE is one, bright shining light in this regard. Read all
We had a fabulous trip! The people were amazing, kind and very professional. The food was exceptionally good! The facilities are beautiful and well kept. We just loved our second time with Rainforest Expeditions and can't wait to go again!! Read all
Fantastic to be deep in the forest surrounded by incredible wildlife with our amazing guide Juan Carlos Yatto, who really knew his stuff! Also his photography skills meant that we could share our experience even better through his telescope using our phone cameras! We learned so much!! He went to extra lengths to find butterflies and moths for us along with so many different birds and monkeys. This was a travel highlight for us as experienced wildlife travellers. We loved our spacious room with no windows and open to more wildlife visitors but after 5 nights the bed could have been a little more comfortable. Luckily we had a second bed to use. Otherwise it was fantastic with hot water showers and fresh drinking water all the time. We were amazed at the variety of food on offer for breakfast, lunch and dinner and couldn’t believe they could have such a wonderful food offering in such a remote location. Even the wine and cocktails were great. I highly recommend spending the extra money and time getting to this location. We stayed at another lodge later which was closer to Puerto Maldonado and there was far less wildlife. Read all
Ederson made the stay at the lodge amazing. I have had many guides around the world and Ederson may be the best I have had across 50 countries. The lodge itself is nice, and has electricity. Food is good, and you really feel immersed in the amazon staying there. Its great that a portion of the money goes back to conservation and research. Noteworthy details: Ederson, the "commander" is the best guide! Read all
Through a travel agency in Lima we booked a 4day/3-night stay at Refugio Amazonas. We were looking for a lodge that was far away from Puerto Maldonado but didn't require almost a day to get there (like TRC), with extensive walking trails and with the principal jungle highlights nearby. We were not disappointed. As we later learned, this is probably the best lodge in the region! Well run, quiet, excellent food, beautifully located in the middle of the forest, everything was "right". Surprisingly there were few mosquitos or bugs. Emperatriz (MP as some call her), our guide, has a keen eye and took us on some unforgettable walks and boat rides, both day and night. The birds at the clay lick were fantastic. The lodge also had evening lectures by young researchers as well as special activities such as collecting night moths and setting a trap camera. We later received videos and photo's of what was found (including a puma!). I can recommend this lodge without reservation. Read all
Abbiamo scelto il Tambopata Research Center perché il nostro obbiettivo era di poter vedere le are da vicino. Ci siamo stati ad inizio dicembre scorso, l'inizio della stagione delle piogge, perciò abbiamo scelto di restare 5 notti/6 giorni, abbiamo messo in conto di perdere delle giornate a causa della pioggia e per la lontananza da Puerto Maldonado, per nostra fortuna durante il nostro soggiorno ha piovuto solo il giorno di partenza proprio quando arrivavamo in aeroporto. Non c'é stato un giorno che non ne abbiamo visto: are di diverse specie e colori e molti altri uccelli e primati, spettacolare l'incontro a pochi centimetri con un formichiere bianco, ma non il giaguaro. Consigliamo di pernottatre almeno 3 notti, il viaggio é molto lungo. Noi eravamo all'aeroporto di Lima già alle 8:00 di mattina e tra un volo in ritardo, uno spostamento in bus di ca. 45 minuti per arrivare al fiume, ed un trasferimento in barca veloce, siamo arrivati al lodge verso le 16:30. All'aeroporto ci ha accolto la nostra guida privata Alfred, che ci ha seguito per tutto il soggiorno, di giorno in giorno abbiamo deciso le escursioni da fare, ed Alfred si é rivelato essere una super guida, un occhio di falco per scoprire anche il più piccolo uccellino, con pazienza é riuscito a farci camminare chilometri nella giungla, ci ha spiegato molto sulla flora e fauna. Bravissimo a far video e foto con i nostri telefoni col suo binocolo e cavalletto che si portava sempre appresso, queste foto sono dei magnifici ricordi. TRC non é un hotel, é un centro di ricerca per le are, in mezzo ad una foresta ed a ore dal primo centro abitato, perciò non avevamo delle grandi aspettative ne sul cibo ne sull'alloggio Read all
This place is a treat!! Incredible flora & fauna, food and service - the staff is quite attentive. Interesting research, with lots of potential. Visiting the Peruvian Amazon Rainforest is a must do! Read all
We had the most amazing time EVER at Refugio Amazonas, and I am so very glad I booked our stay here. Honestly, this is to date probably the best travel experience we have ever had and I will never forget our time in the Amazon here. First of all, the lodge is beautiful. Our room was lovely. The beds were comfy. The bathrooms are large. It is incredible waking up to the sounds of the jungle and falling asleep to the insects at night. We did have some insects in our room, and a rodent, and even a tarantula (non poisonous)! But it was part of the experience. The food was great and they prioritize native and local ingredients. My partner and I are both vegan and they took SUCH good care of us. The food was delicious, and we had ample vegan options every meal. Everything from the first email contacts with Katia to the transfers to the first night info session to the activities-- which I will get to in a second-- was incredible. It is a bit pricey, but everything is included and there is nothing you need to worry about, and it was so worth it. OK now for the best part-- the activities. Holy wow. First of all, we had the best guide EVER-- Juan Carlos. He was amazing. He is a bird expert, knows so much about the animals who live in the rainforest, the insects, the trees, the plant life, it was incredible. He also so clearly cared that everyone in our group had an amazing time. He took so many pictures for us, pointed out so many animals for us, and made sure we all enjoyed every moment. I can’t thank him enough because it made our time here so very special. We went on many activities that were amazing, and we saw so many animals— capybaras, howler monkeys, capuchin monkeys, so many incredible insects, frogs, tarantulas, so many many birds and butterflies. Every activity was amazing but the highlights were the macaw clay lick and the canopy tower. And we were super impressed and happy to hear about their commitment to conservation, protecting the animals, and supporting the native community nearby. There is so much I could say about our stay here but I would recommend a 3 night stay over 2 nights (so you can do more activities), and make sure you read the guides they send via email before you come so you pack correctly and know what to expect and bring--the guide includes everything so that there are no surprises, and we knew exactly what to expect, although our stay exceeded our expectations! I highly, highly recommend staying here, it was magical, it was a once in a life time adventure, and I will never forget it. I also want to shout out the manager of the lodge, I forgot her name, but she was incredible, as well as the service staff at meal times, who were so kind and helpful to point out what was vegan all of the time. I truly hope we can come back some day!! Read all
The lodge was kept very clean, and the staff paid close att to people's needs. It was very easy find activities and the guides were excellent with a genuine intended approach towards sustainability and environment longevity. Read all
A superb stay in a top class Lodge which gave a wonderful insight into the Amazon wildlife. Our guide was Juan Carlos who has incredible knowledge and a lovely sense of humour. The staff in the lodge were so friendly and the meals provided were something to look forward to. A wonderful experience! Read all
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